A dominant mutation in Escherichia coli OmpR lies within a domain which is highly conserved in a large family of bacterial regulatory proteins
Summary
We have fortuitously created an in-frame insertion mutation in the cloned ompR gene of Escherichia coli in the course of an experiment involving linker insertion mutagenesis. According to the DNA sequence, the mutant protein has an insertion at the 53rd amino acid residue, which replaced the original valine, with the sequence Ala-Leu-Glu. The expression level of the mutant protein, OmpRX6, in a minicell system, is similar to that of the wild-type protein and the size of the mutant is slightly larger than the wild type by approxiately 300 daltons. This mutant was completely unable to activate porin expression as the wildtype does, and in addition, this phenotype was shown to be dominant over the wild type. Comparison of the amino acid sequence of OmpRX6 with those of a family of homologous bacterial regulatory proteins revealed that the mutation lies in a domain which is highly conserved among these proteins.
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References
Comeau DE, Ikenaka K, Tsung K, Inouye M (1985) Primary characterization of the protein products of the Escherichia coli ompB locus: Structure and regulation of synthesis of the OmpR and EnvZ proteins. J Bacteriol 164:578–584
Hall MN, silhavy TJ (1981a) The ompB locus and the regulation of the major outer membrane proteins of Escherichia coli K-12. J Mol Biol 146:23–43
Hall MN, silhavy TJ (1981b) Genetic analysis of the ompB locus in Escherichia coli K-12. J Mol Biol 151:1–15
Nara F, Matsuyama S, Mizuno T, Mizushima S (1986a) Molecular analysis of mutant ompR genes exhibiting different phenotypes of osmoregulation of the ompF and ompC genes of Escherichia coli. Mol Gen Genet 202:194–199
Nara F, Mizuno T, Mizushima S (1986b) Complementation analysis of the wild-type and mutant ompR genes exhibiting different phenotypes of osmoregulation of the ompF and ompC genes of Escherichia coli. Mol Gen Genet 205:51–55
Norioka S, Ramakrishnan G, Ikenaka K, Inouye M (1986) Interaction of a transcriptional activator, OmpR, with reciprocally osmoregulated genes, ompF and ompC, of Escherichia coli. J Biol Chem 261:17113–17119
Pirhonen M, Soarilahti HT, Kerkela S, Palva ET (1986) In vivo cloning and characterization of mutations of the regulatory locus ompR of Escherichia coli K12. Mol Gen Genet 203:520–523
Pollitt S, Zalkin H (1983) Role of primary structure and disulfide bond formation in beta-lactamase secretion. J Bacteriol 152:27–32
van Alphen W, Lugtenberg B (1977) Influence of osmolarity of the growth medium on the outer membrane protein pattern of Escherichia coli. J. Bacteriol 131:623–630
Winans SC, Ebert PR, Stachel SE, Gordon MP, Nester EW (1986) A gene esential for Agrobacterium virulence is homologous to a family of positive regulatory loci. Proc Natl Acad Sci USA 83:8278–8282
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Author notes
Kazuhiro Ikenaka
Present address: Institute for Protein Research, Osaka University, 3-2 Yamada-oka, 565, Suita, Osaka, Japan
Authors and Affiliations
UMDNJ— Robert Wood Johnsen Medical School in Piscataway, G75 Hoes Lane, 08854, Piscataway, NJ, USA
Kangla Tsung
Department of Biochemistry, State University of New York at Stony Brook, 11794, Stony Brook, NY, USA
Dorothy E. Comeau & Masayori Inouye
Authors
Kazuhiro Ikenaka
Kangla Tsung
Dorothy E. Comeau
Masayori Inouye
Additional information
Communicated by K. Isono
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Cite this article
Ikenaka, K., Tsung, K., Comeau, D.E. et al. A dominant mutation in Escherichia coli OmpR lies within a domain which is highly conserved in a large family of bacterial regulatory proteins. Molec. Gen. Genet.211, 538–540 (1988). https://doi.org/10.1007/BF00425713
Received: 24 March 1987
Issue date: March 1988
DOI: https://doi.org/10.1007/BF00425713
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